Aims: To determine whether there is a possibility of contamination of Fresh Frozen Plasma bags by the organism Pseudomonas aeruginosa during the process of thawing.
Study design: The study employed in the research is in an experimental approach.
Place and Duration of Study: Daniel Mercado Medical Center (DMMC) University of Perpetual Help - DJGTMU, between September 2018 and October 2018.
Methodology: Live colonies of P. aeruginosa is from a Cetrimide slant were directly inoculated in a water bath with distilled water in order to stimulate an environment where a possible water contamination of P. aeruginosa is present. To confirm the possibility of a bacterium to pass through a frozen blood bag during thawing process, 10 FFP blood bags were submerged and thawed in the water bath for 1 hour at 37C. After thawing, each of the blood bags were labelled as blood bag A, B, C, D, E, F, G, H, I, and J. 5ml of thawed FFP was aspirated from each and was streaked on Blood Agar Plate (BAP), MacConkey Agar (MAC), and Cetrimide Agar and were incubated at 37 degrees C for 3 days. All blood bags were tested for two trials each. Culture media plates were labelled as A1, A2, Bl, B2 up to J1 and J2 in order to make the research more viable.
Results: Out of the 20 trials done, 4 trials showed visible colony growth in MAC agar, exhibiting green pigmentation thus yielded a positive identification of P. aeruginosa in Cetrimide agar. To further confirm the identification of the growth of colonies in Cetrimide agar, The BBL CRYSTAL Enteric/Nonfermenter (E/NF) Identification (ID) System was used to determine the biochemical reactions of each sample. The samples yielded a positive ID of the organism Pseudomonas aeruginosa.
Conclusion: There were two out of ten blood bags that tested positive for the presence of P. aeruginosa after thawing at 37 degrees C for 1 hour. The contamination of the FFP blood bags might have been caused by cracks on the blood bag due to its storage in extremely low temperatures prior to thawing. Also, the FFP units tested positive might have been contaminated initially prior to thawing.